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There is almost no scientific evidence to suggest ecdysterone has any effects in humans. Whilst laboratory studies show ecdysterone raises protein synthesis levels in muscle cells, this failed to translate into strength gains in the single human study that has been performed (6). There has only been one study performed on the reputed testosterone boosting properties of ecdysterone in humans, and this did not produce any change in testosterone levels or body composition (4). Additionally, ecdysterone had no effect on lipid metabolism or liver enzymes in male athletes (6). It is anecdotally thought that the concentration of ecdysterone used in these studies may have been too low to see any effect. A great deal more study is needed before conclusions can be drawn.

Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more [38] or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram. [39] [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography . [2] : 10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. [39]

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